首页/产品中心/pCMV-Nsf(mouse)-3×FLAG-mCMV-TurboRFP-Neo
Description
产品说明
pCMV-Nsf(mouse)-3×FLAG-mCMV-TurboRFP-Neo 是一款专为 哺乳动物细胞 设计的 基因表达 型载体，主要用于 蛋白过表达 研究。该载体携带 Kan 抗性标记，并携带 筛选标记 Neo/G418、红色 荧光蛋白、FLAG 标签，基因片段来源于小鼠。适用于 ORF 片段的高效表达，是 基因表达 领域理想的实验工具。淼灵生物对该载体进行了严格的质控验证（包括酶切图谱分析及测序鉴定），确保每批次产品均具备高纯度、超螺旋结构完整及低内毒素特性，转染效率稳定。同时，我们提供全程技术支持与极速发货服务，为您的实验节省宝贵时间。
| 所属分类 | 质粒载体 |
|---|---|
| 品牌 / 来源 | CS-BIC | 中科生信 |
| 最近更新 | 2026-10-18 |
| 别称 | NM_008740.4;AI316878; AU020090; AU067812; SKD2 |
| 启动子 | CMV |
| 基因长 | 2235bp |
| 复制子 | pUC |
| 感受态 | DH5a |
| 拷贝数 | 高 |
| 原核抗性 | Kan |
| 参考货期 | 现货 |
| 培养温度 | 37度 |
| 正向引物 | CMV-F |
| 片段物种 | 小鼠 |
| 片段类型 | ORF |
| 筛选标记 | Neo/G418 |
| 荧光标记 | 红色 |
| 蛋白标签 | FLAG |
| 质粒宿主 | 哺乳细胞 |
| 质粒序列 | tagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctggtttagtgaaccgtcagatccGCTAGCGCTACCGGACTCAGATCTCGAGCTCCGAATTCGCCACCatggcgggccggactatgcaagctgcgagatgccctactgatgaattatctttaagcaactgtgcggttgtgaacgaaaaagatttccagtctggccagcatgtgatggtgaggacgtcccccaatcacaagtacatatttacgctgaggacccatccgtcagtggttccaggatgcatcgcgttcagcttgcctcagcgaaaatgggctggactttctattggacaggacatagaagttgccttgtattcgtttgacaaagccaagcagtgcatcggcacaatgaccatcgagatcgacttcctgcagaagaagaacatcgactccaacccttacgacaccgacaagatggccgccgagttcatccagcagttcaacaaccaggccttctcagtggggcagcagcttgtctttagcttcaatgataagctctttggattactggtgaaggacattgaagccatggatcccagcatcctgaagggagagcctgcatcaggcaaaaggcagaagattgaggtaggcctggttgttggaaacagtcaagttgcatttgaaaaagcagaaaattcgtcgctcaatcttatcggcaaagctaaaaccaaggaaaatcgccagtccatcatcaatcccgactggaactttgagaaaatggggatcggcggtctggataaggagttctcggacattttccgaagagcatttgcttctcgggtgttccccccggagatcgtggaacagatgggttgcaaacatgttaaaggcatcctgttatacggaccccctggttgtggtaaaactctcttggctcgacagattggcaagatgctgaatgcgagagagcccaaggtggtcaatgggccagaaatccttaacaagtatgtgggagaatcagaggctaacattcgtaaactctttgctgatgccgaagaggagcaaaggaggcttggtgctaacagtggcttgcacatcatcatctttgatgaaatcgatgccatctgcaagcagagagggagcatggccggcagcactggagtgcatgacaccgtggtcaaccagctgctgtccaagatcgatggcgttgagcagctgaacaacatcctcgttatcggaatgaccaatagaccagatttgatagacgaagctctccttcgacccggaagactggaagttaaaatggagataggcttgccagatgagaagggtcgactccagatccttcatatccacacagcaaggatgagagggcaccagttactgtctgcagatgtggacatcaaggagctggctgtggagactaagaatttcagtggcgctgagctggagggtctggtgcgagcagcgcagtccacagccatgaacagacatatcaaggccagcaccaaagtagaagtagacatggagaaggcagagagcctgcaggtgacaagaggggacttccttgcttccctggagaacgatatcaaaccagcatttggcaccaaccaagaggattatgcaagttacattatgaatgggatcatcaagtggggtgaccccgtgacccgagtcctagatgacggagagctgctggtccagcaaacgaaaaacagtgaccggacaccactcgttagtgtcctcctagaagggcctcctcacagcgggaagactgccctggctgcaaagattgccgaggaatccaacttccccttcatcaagatctgctcacctgataagatgattggcttctccgagacagccaagtgtcaggccatgaagaagatctttgatgatgcttacaaatctcagctcagctgtgtggtcgtggatgacattgagaggctgctcgattatgtccccattggtccccggttctccaacctggtattgcaggctcttcttgtgttactgaaaaaggcgcctcctcagggccgcaagcttcttatcatcgggaccaccagccgcaaagatgtcctgcaggaaatggagatgctgaacgccttcagcaccaccatccacgtgcccaacattgccacaggagagcagctgctggaagctctggagcttttgggcaacttcaaagataaggaacgcaccacaattgctcagcaagtcaaagggaagaaagtctggataggaatcaagaagttactaatgttgattgagatgtccctgcagatggatcctgagtaccgtgtgaggaaattcttggccctcatgagagaagaaggcgctagtcccctggactttgatAAGCTTACGCGTGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGACTACAAGGATGACGATGACAAGTGAGGATCCGGGCCCGTTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGTCTAGAGATATACTGAGTCATTAGGGACTTTCCAATGGGTTTTGCCCAGTACATAAGGTCAATAGGGGTGAATCAACAGGAAAGTCCCATTGGAGCCAAGTACACTGAGTCAATAGGGACTTTCCATTGGGTTTTGCCCAGTACAAAAGGTCAATAGGGGGTGAGTCAATGGGTTTTTCCCATTATTGGCACGTACATAAGGTCAATAGGGGTGAGTCATTGGGTTTTTCCAGCCAaTTTAATTAAAACGCCATGTACTTTCCCACCATTGACGTCAATGGGCTATTGAAACTAATGCAACGTGACCTTTAAACGGTACTTTCCCATAGCTGATTAATGGGAAAGTACCGTTCTCGAGCCAATACACGTCAATGGGAAGTGAAAGGGCAGCCAAAACGTAACACCGCCCCGGTTTTCCCCTGGAAATTCCATATTGGCACGCATTCTATTGGCTGAGCTGCGTTCTACGTGGGTATAAGAGGCGCGACCAGCGTCGGTACCGTCGCAGTCTTCGGTCTGACCACCGTAGAACGCAGATCaccggtcgccaccATGAGCGAGCTGATCAAGGAGAACATGCACATGAAGCTGTACATGGAGGGCACCGTGAACAACCACCACTTCAAGTGCACATCCGAGGGCGAAGGCAAGCCCTACGAGGGCACCCAGACCATGAAGATCAAGGTGGTCGAGGGCGGCCCTCTCCCCTTCGCCTTCGACATCCTGGCTACCAGCTTCATGTACGGCAGCAAAGCCTTCATCAACCACACCCAGGGCATCCCCGACTTCTTTAAGCAGTCCTTCCCTGAGGGCTTCACATGGGAGAGAATCACCACATACGAAGACGGGGGCGTGCTGACCGCTACCCAGGACACCAGCTTCCAGAACGGCTGCATCATCTACAACGTCAAGATCAACGGGGTGAACTTCCCATCCAACGGCCCTGTGATGCAGAAGAAAACACGCGGCTGGGAGGCCAACACCGAGATGCTGTACCCCGCTGACGGCGGCCTGAGAGGCCACAGCCAGATGGCCCTGAAGCTCGTGGGCGGGGGCTACCTGCACTGCTCCTTCAAGACCACATACAGATCCAAGAAACCCGCTAAGAACCTCAAGATGCCCGGCTTCCACTTCGTGGACCACAGACTGGAAAGAATCAAGGAGGCCGACAAAGAGACCTACGTCGAGCAGCACGAGATGGCTGTGGCCAAGTACTGCGACCTCCCTAGCAAACTGGGGCACAGATAAagcggccgcgactctagatcataatcagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttaaggcgtaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtcctgaggcggaaagaaccagctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaagatcgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgacgcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccctagggggaggctaactgaaacacggaaggagacaataccggaaggaacccgcgctatgacggcaataaaaagacagaataaaacgcacggtgttgggtcgtttgttcataaacgcggggttcggtcccagggctggcactctgtcgataccccaccgagaccccattggggccaatacgcccgcgtttcttccttttccccaccccaccccccaagttcgggtgaaggcccagggctcgcagccaacgtcggggcggcaggccctgccatagcctcaggttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgccatgcat 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| 质粒用途 | 基因表达 |
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